0f1de4b551b0
Update
author | Steve Losh <steve@stevelosh.com> |
---|---|
date | Mon, 24 Feb 2020 23:56:15 -0500 |
parents | 0e18286c1a1a |
children | 31f98ce9303a |
branches/tags | (none) |
files | README.markdown |
Changes
--- a/README.markdown Mon Feb 24 20:40:34 2020 -0500 +++ b/README.markdown Mon Feb 24 23:56:15 2020 -0500 @@ -1006,3 +1006,10 @@ genome but it complains that it requires the genome to be uncompressed. Tried to trick it into working by psubbing `zcat` but that didn't work. Gave up and decompressed the damn reference. + +Once the genome index was built, started aligning the reads. It failed right +away complaining about a zero-length read. `zcat | grep`ing showed the read as +being there in both files, *but* I bet this was an issue of the pairs getting +out of sync because we grepped out the overrepresented sequences naively. Tried +restarting the alignment on the original data and it's still going, so that's +probably it. Need to figure out how to filter those bad reads properly tomorrow.