# HG changeset patch # User Steve Losh # Date 1698696783 14400 # Node ID ef2318c8284e5bd7db68b7e013152f2113aa4951 # Parent cc1cb318ef3289539330de82c65a6b2848474989 Update diff -r cc1cb318ef32 -r ef2318c8284e README.markdown --- a/README.markdown Sat Oct 28 23:35:03 2023 -0400 +++ b/README.markdown Mon Oct 30 16:13:03 2023 -0400 @@ -556,3 +556,29 @@ Started digging into the HG545 paper. I hate that we only have 2 classes for this one, it feels far too rushed. + +## 2023-10-29 + +Finished the second readthrough of the paper. I feel a little better now, but +am still not perfectly sure on the questions. + +## 2023-10-30 + +HG545 class, mostly on DNA repair. Makes the case that a lot of DNA repair +reuses the same polymerases (e.g. δ) as replication (but not all, e.g. +single-base repair uses something else (I think β?)), and you can kind of think +of it as just another form of replication. + +Another important point: replication is not deterministic, because it doesn't +have to be. As long as the whole genome (well, chromosome) gets replicated, it +doesn't matter precisely where that replication started. This is very different +from e.g. transcription, because starting in a different place would transcribe +a different gene. + +Also important to define terms like lesion vs mutation: a lesion is detectable +DNA damage, which then get fixed into place (in the "use fixer on the photo +paper" sense, not the "repair" sense) by subsequent replication. + +I think I'm feeling a little better about the homework questions now after +talking through it a bit. Need to get some rough drafts sketched out for when +I meet with the study group tomorrow.