# HG changeset patch # User Steve Losh # Date 1584642039 14400 # Node ID 5b99f3af544cecf0dc6a11844fa417743e5e9f69 # Parent 882a8f9832f42c5b03e6bde59eadd11a823956a8 Update diff -r 882a8f9832f4 -r 5b99f3af544c README.markdown --- a/README.markdown Thu Mar 19 11:03:01 2020 -0400 +++ b/README.markdown Thu Mar 19 14:20:39 2020 -0400 @@ -1158,3 +1158,17 @@ Plan for today: rerun the trimming with the 5 base requirement and using the 1 mismatch deduplication data as the source, and then kick off an alignment if I still have time (unlikely). + +Neomutt has suddenly started shitting the bed and failing to quite with `Unable +to write` errors. I *hate* software. Tried updating to the latest `master` and +rebuilding. I guess we'll see if it fixes things. + +Trimming finished. It looks like increasing the minimum adapter length to +5 fixed the base imbalance issue. Good. + +Decided to rebuild the STAR index I had made because I can't remember whether +I had already rebuilt it with the different reference file. Shouldn't take too +long. + +Index rebuild finished in ~35 minutes. Kicked off the 26 alignment runs. That +should take a lot longer.